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R&D Systems
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Image Search Results
Journal: Cytokine
Article Title: IL-33/ST2 enhances MMP-12 expression by macrophages to mediate inflammatory and immune response in IgG4-Related Ophthalmic Disease.
doi: 10.1016/j.cyto.2024.156754
Figure Lengend Snippet: Fig. 2. Expression of IL-33/ST2 in IgG4-ROD A: Immunohistochemistry anal- ysis of IL-33、ST2 in IgG4-ROD lacrimal gland tissues relative to normal tissues. B: Statistical analysis of the immunohistochemistry results from 9 IgG4-ROD patients with lacrimal gland involvement and 9 normal lacrimal gland tis- sues. Normal: lacrimal gland prolapse patients. (*, p < 0.05).
Article Snippet: Antibodies used for immunohistochemical analysis included IL-33 (Proteintech, Catalog no.: 66235–1-Ig),
Techniques: Expressing, Immunohistochemistry
Journal: PLoS Medicine
Article Title: An Immune Basis for Lung Parenchymal Destruction in Chronic Obstructive Pulmonary Disease and Emphysema
doi: 10.1371/journal.pmed.0010008
Figure Lengend Snippet: (A) CD14 + , lymphocyte-depleted lung leukocytes were cultured with and without the indicated amounts of recombinant human IP-10 and IFN-γ, and supernatants were assessed for the presence of MMP12 by Western blotting. (B) Fold increase relative to unstimulated of MMP12 mRNA from lung macrophages stimulated without ( – ) and with ( + ) 500 ng/ml of IP-10 in the presence or absence of a function-blocking antibody to CXCR3 as determined by real-time PCR. (C and D) Lung tissue from a participant with emphysema (C) shows strong immune staining for MMP12 localized to macrophages (arrows), and (D) shows lung tissue from a control participant without emphysema and with undetectable MMP12. The insets show a high-power view of lung macrophages staining positive (C) and negative (D) for MMP12 (×60) *, p = 0.04.
Article Snippet: Supernatants were collected, and MMP12 was detected using
Techniques: Cell Culture, Recombinant, Western Blot, Blocking Assay, Real-time Polymerase Chain Reaction, Staining, Control